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KMID : 0545120150250111936
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Journal of Microbiology and Biotechnology
2015 Volume.25 No. 11 p.1936 ~ p.1943
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An Environmentally Friendly and Efficient Method for Extraction of PHB Biopolymer with Non-Halogenated Solvents
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Aramvash Asieh
Gholami-Banadkuki Narges
Moazzeni-Zavareh Fatemeh
Hajizadeh-Turchi Samira
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Abstract
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The present study developed an efficient and environmentally friendly method for recovering polyhydroxybutyrate (PHB) from Cupriavidus necator. Several non-halogenated solvents were tested and it was found that butyl acetate and ethyl acetate are powerful solvents for the biopolymer. Testing was performed to examine the effects of temperature (25°C until temperature below solvent boiling points) and heating incubation time (0-60 min) on the two solvents. Butyl acetate had a higher recovery level (96%) and product purity (up to 99%) than ethyl acetate at 103°C and a heating incubation time of 30 min. Under these conditions, PHB recorded the highest molecular weight of 1.4 × 106 compared with the standard procedure (i.e., recovery using chloroform). The proposed strategy showed that butyl acetate is a good alternative to halogenated solvents such as chloroform for recovery of PHB.
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KEYWORD
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Butyl acetate, Design expert, Central composite design, Ethyl acetate, Cupriavidus necator, Response Surface methodology
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